See NucView™ 488 Caspase-3 Enzyme Substrate in action in this video provided by Essen Bioscience using their IncuCyte ZOOM™ live cell imaging system. Cells from the HT-1080 human fibroscarcoma-derived cell line were treated with 150 nM camptothecin in the presence of 5 uM NucView™ 488 Caspase-3 Substrate. The cells were imaged every 30 minutes for 24 hours, during which apoptosis can be observed by changes in cellular morphology and green fluorescence that is emitted upon DNA binding from the DNA binding NucView™ 488 free dye released by the activity of Caspase-3 upon the substrate.
DEVD-NucView™ Caspase-3 Substrates are a novel fluorogenic substrate developed by Biotium to follow caspase-3 activity in intact cells in real-time. We have invented a whole new concept to design fluorogenic enzyme substrates by attaching an enzyme substrate moiety to a functional dye, thereby making the functional dye nonfunctional. Upon enzymatic cleavage of the substrate, the dye restores its functionality and thus becomes capable of binding to its target to emit fluorescence.
Green fluorogenic NucView™ 488 Caspase-3 Substrate can be detected in the FITC channel by fluorescence microscopy, flow cytometry, or fluorescence microplate reader. Detection of apoptosis using NucView™ 488 Caspase-3 Substrate has been reported in more than 90 different primary and immortalized cells types in more than