Cy3 Cy5 荧光染料
 

Cy3 NHS Ester

 

Structure

MW = 765.95

Structure shown as mono-functional NHS ester.

Spectra

Excitation and emission spectra of unconjugated Cy3 dye in phosphate buffered saline.

Properties
Excitation maximum (nm)
548
Emission maximum (nm)
562
Extinction coefficient (M-1 cm-1)
150 000 *
Quantum yield
0.04 *
Fluorescence lifetime (ns)
<0.3

* Measured in phosphate-buffered saline, Mujumdar, R. B., Ernst, L. A., Mujumdar, S. R., Lewis, C. J., and Waggoner, A. S. (1993) Bioconjugate Chem. 4, 105-111.

(Note: The quantum yield of Cy3 is significantly increased when in a rigid microenvironment, for example in highly viscous solvents, or when conjugated to biomolecules).

Upon attachment to biomolecules (for example oligonucleotides), negligible shifts in excitation and emission wavelength have been observed. In addition no effects on these spectral characteristics have been observed in the presence of 10% DMSO or under a range of pH conditions.

 

Cy5 NHS Ester

Structure

MW = 791.99

Structure shown as mono-functional NHS ester.

Spectra

Excitation and emission spectra of unconjugated Cy5 dye in phosphate buffered saline.

Properties
Excitation maximum (nm) 646
Emission maximum (nm) 664
Extinction coefficient (M-1 cm-1) 250 000 *
Quantum yield 0.27 *
Fluorescence lifetime (ns) 1.0

* Measured in phosphate-buffered saline, Mujumdar, R. B., Ernst, L. A., Mujumdar, S. R., Lewis, C. J., and Waggoner, A. S. (1993) Bioconjugate Chem. 4, 105-111.

Upon attachment to biomolecules (for example oligonucleotides), negligible shifts in excitation and emission wavelength have been observed. In addition no effects on these spectral characteristics have been observed in the presence of 10% DMSO or under a range of pH conditions.

编号
产品名
规格
单价(rmb)
产地
生产厂商
OPA13101 Cy3 NHS ester(high purity)
1mg
¥1,990
中国
OPE TECH
OPA15101 Cy5 NHS ester(high purity)
1mg
¥1,990
中国
OPE TECH
           
OPE3010 CyDye DIGE Fluor, Cy3 minimal dye
10 nmole
¥5,500
中国
OPE TECH
OPE5010 CyDye DIGE Fluor, Cy5 minimal dye
10 nmole
¥5,500
中国
OPE TECH

Copyright © 上海开放科技发展有限公司

电话:021-61610870 61610871