NAO is a mitochondrial dye whose staining is not dependent on mitochondrial membrane potential,unlike JC-1 (70011) and Rhodamine 123 (70010). NAO has been used to study multidrug resistanceand to measure changes in mitochondrial mass during apoptosis (3).
λEx/λEm (MeOH) = 495/522nm
ε (MeOH) = 63,000
Orange solid soluble in DMSO or DMF
Store at 4°C and protect from light, especially in solution