This affinity purified antibody is directed against the 6X His epitope and is useful in determining its presence in over expressed proteins in various assays. The antibody recognizes the 6X His epitope tag (His-His-His-His-His-His) fused to either the amino- or carboxy- termini of targeted proteins in transfected or transformed cells. This product is prepared by labeling high quality monoclonal mouse anti-6X His IgG (H+L) with the deep-red fluorescent dye CF594. CF594 is spectrally similar to Alexa Fluor? 594 and Texas Red? dye but yields much brighter protein conjugates due to its high quantum yield and exceptional water solubility. CF594 also has excellent photostability ideal for demanding applications, such as confocal microscopy and single molecule imaging. The dye is particularly useful to combine with our blue fluorescent CF350, green fluorescent CF488A and far red CF633 or CF647 for multi-color imaging.
