Polyclonal anti-V5 (GKPIPNPLLGLDST) is designed to detect V5 tagged fusion proteins. The V5 epitope tag is derived from a small epitope (Pk) present on the P and V proteins of the paramyxovirus of simian virus 5 (SV5). The antibody recognizes the epitope tag fused to either the amino- or carboxy- termini of targeted proteins. This product is prepared by labeling high quality polyclonal rabbit anti-V5 IgG (H+L) with the far-red fluorescent dye CF640R. CF640R is a novel rhodamine-based dye spectrally similar to Cy?5 and Alexa Fluor? 647. CF640R is much brighter than Cy?5 and at least as bright as Alexa Fluor? 647 when excited at 633 or 635 nm. However, the most important advantage of CF640R is its exceptional photostability. CF640R is far more photostable than the cyanine-based dyes Cy?5 and Alexa Fluor? 647. It is also more photostable than Atto 647N, a dye that has been extensively used in single molecule-based imaging. CF640R is also extremely water-soluble and pH-insensitive. These properties make CF640R ideally suited for demanding applications such as confocal microscopy. Please download the CF640R dye flyer for more details.
