This product is prepared by labeling highly cross-adsorbed donkey anti-rabbit IgG (H+L) with the near-IR fluorescent dye CF750, one of an outstanding series of CF dyes developed by Biotium. To minimize cross-reactivity, the antibody has been adsorbed against bovine, chicken, goat, guinea pig, Syrian hamster, horse, human, mouse, rat, and sheep serum. Our CF750-labeled antibodies are suitable for flow cytometry with 633 nm excitation. Although the absorption peak of the conjugate is at 755 nm, excitation at 633 or 635 nm still yields excellent fluorescence. In fact, our CF750 antibody conjugates are brighter and more specific than antibodies labeled with APC-Alexa Fluor? 750 tandem dye (see the near-IR CF flyer). CF750 is one of our three near-IR CF dyes. It is superior to other similar near-IR dyes, such as Alexa Fluor? 750, IRDye? and the DyLight? near-IR dye. All of our near-IR CF dyes have combined advantages in brightness, photostability, specificity and novel features ideal for in vivo imaging, near-IR Western blotting and flow cytometry. Please also see CF750 NHS ester (cat#92142) and VivoBrite antibody labeling kit (cat#92161) for carrying out your own protein labeling.
