CF dye goat anti-mouse IgG isotype-specific secondary antibody conjugates are labeled with our exceptionally bright, photostable CF dyes. The conjugates are prepared from affinity-purified antibodies that react with Fc portion of the heavy chain of mouse IgG2b. To minimize cross reactivity, the antibodies are cross-adsorbed against other mouse IgG subclasses (IgG1, IgG2a, IgG2c, IgG3), and human, bovine and rabbit serum proteins.
Our CF750-labeled antibodies are suitable for flow cytometry with 633 nm excitation. Although the absorption peak of the conjugate is at 755 nm, excitation at 633 or 635 nm still yields excellent fluorescence. In fact, our CF750 antibody conjugates are brighter and more specific than antibodies labeled with APC-Alexa Fluor? 750 tandem dye (see the Near-IR CF flyer).