CF405M Annexin V conjugate is Annexin V labeled with our superior blue fluorescent dye CF405M. Annexin V is a 35-36 kDa protein that has a high affinity for phosphatidylserine (PS). In normal viable cells, PS is located on the cytoplasmic surface of the cell membrane. In apoptotic cells, however, PS is translocated from the inner to the outer leaflet of the plasma membrane, making itself available for Annexin V binding.
CF405M (Ex/Em = 408/452 nm) is a blue fluorescent dye spectrally similar to Pacific Blue? dye. CF405M is as bright as Pacific Blue dye but is significantly more photostable than the latter. An additional advantage of CF405M is that it has less spillover in the 525/50 green channel than Pacific Blue? dye due to narrower emission peak, making CF405M a superior choice in multi-color detection applications.