PAGE GelRed® is a non-toxic, non-mutagenic red DNA gel stain specifically designed to stain DNA in polyacrylamide gels. PAGE GelRed® can be imaged using a 254 nm UV transilluminator with an ethidium bromide filter. While PAGE GelRed® also can be used to stain DNA in agarose gels, Biotium’s original GelRed® nucleic acid gel stain (catalog number 41003) is more sensitive for agarose gel applications. PAGE GelRed® can be removed from DNA after agarose gel staining using commonly available gel extraction kits. PAGE GelRed® is 6-8 times more sensitive for dsDNA over RNA.
PAGE GelRed® was subjected to a series of tests at Biotium and two independent laboratories to assess the dye’s safety for routine handling and disposal. Laboratory tests show that the dye is impenetrable to latex or nitrile gloves and cell membranes. Unlike the highly mutagenic EtBr and the reportedly mutation-enhancing SYBR® Green I (1), PAGE GelRed® is non-toxic and non-mutagenic in bacterial AMES tests at concentrations well above the working concentrations used in gel staining. PAGE GelRed® successfully passed environmental safety tests in compliance with CCR Title 22 Hazardous Waste Characterization, under which it is classified as non-hazardous waste. A complete safety report is available under the downloads tab.
PAGE GelRed® is available as a ready-to-use staining solution at 1X in water, or a concentrated dye solution at 10,000X in water.
Product / Method
DNA prestaining with GelRed® Prestain Plus 6X DNA Loading Dye
GelRed® loading buffer is added directly to the DNA sample before loading
• Fast & simple: one-step sample loading & DNA staining
• Not recommended for PAGE, DGGE, EMSA, or PFGE gels
• Routine agarose gels
Precast gel staining with GelRed® 10,000X in water
GelRed® is mixed with molten agarose before gel casting
Familiar protocol, rapid results
Precast gel staining with GelRed® Agarose LE
Agarose is supplied pre-coated with GelRed®, just dissolve, heat, and pour
Safer & more convenient, no need to handle concentrated dye
No fluorescent dye is added to the gel, it is stained in 3X GelRed® solution after electrophoresis
• Most accurate sizing/sharpest bands
Extra staining step (up to 30 minutes) after electrophoresis (some customers report good results after only 5 minutes if dye is not reused)
• Highly accurate band sizing
Post-electrophoresis gel staining of PAGE gels using PAGE GelRed® 10,000X or 1X in water
No fluorescent dye is added to the gel, it is stained in 1X PAGE GelRed® solution after electrophoresis
• Formulated for efficient penetration and staining of polyacrylamide gels
Extra staining step of approx. 30 minutes after electrophoresis
Staining of nucleic acids in PAGE gels