Product / Method
DNA prestaining with GelRed® Prestain Plus 6X DNA Loading Dye
GelRed® loading buffer is added directly to the DNA sample before loading
• Fast & simple: one-step sample loading & DNA staining
• Not recommended for PAGE, DGGE, EMSA, or PFGE gels
• Routine agarose gels
Precast gel staining with GelRed® 10,000X in water
GelRed® is mixed with molten agarose before gel casting
Familiar protocol, rapid results
Precast gel staining with GelRed® Agarose LE
Agarose is supplied pre-coated with GelRed®, just dissolve, heat, and pour
Safer & more convenient, no need to handle concentrated dye
No fluorescent dye is added to the gel, it is stained in 3X GelRed® solution after electrophoresis
• Most accurate sizing/sharpest bands
Extra staining step (up to 30 minutes) after electrophoresis (some customers report good results after only 5 minutes if dye is not reused)
• Highly accurate band sizing
Post-electrophoresis gel staining of PAGE gels using PAGE GelRed® 10,000X or 1X in water
No fluorescent dye is added to the gel, it is stained in 1X PAGE GelRed® solution after electrophoresis
• Formulated for efficient penetration and staining of polyacrylamide gels
Extra staining step of approx. 30 minutes after electrophoresis
Staining of nucleic acids in PAGE gels
Note: An improved version of this product is available. GelRed® Prestain Plus 6X DNA Loading Dye has been reformulated formulated to increase brightness and minimize DNA migration shift.
GelRed® is a sensitive, stable and environmentally safe fluorescent nucleic acid dyes designed to replace the highly toxic ethidium bromide (EtBr). 6X GelRed® Prestain Loading Buffers are gel loading buffers containing density agents, tracking dyes, and GelRed® dye. The 6X prestain loading buffer is added to samples in place of gel loading buffer, and eliminates the need to add fluorescent DNA dye to the agarose gel during casting. Download the product protocol on this page for a comparison of prestain, precast, and post-stain protocols for GelRed®, and to find the recommended method for your application.
For detailed information, please visit the DNA Stain Technology page.
The 6X GelRed® Prestain Loading Buffers are available with blue tracking dyes or with orange tracking dye. Prestain buffer with blue tracking dyes (cat. no. 41009) contains two blue electrophoresis tracking dyes that run at approximately 1.5 kb and 200 bp in a 1% agarose gel. Prestain buffer with orange tracking dye (cat. no. 41010) contains an orange electrophoresis tracking dye that runs at approximately 50 bp in a 1% agarose gel.
GelRed® and EtBr have virtually the same spectra, so you can directly replace EtBr with GelRed® without changing your existing imaging system. In addition, GelRed® is far more sensitive than EtBr, which cannot be used in DNA loading buffer to prestain DNA. GelRed® is compatible with downstream applications such as sequencing and cloning. GelRed® is efficiently removed from DNA by commercial gel extraction kits or by phenol/chloroform extraction and ethanol precipitation.
This product is a replacement for catalog no. 31010.