GelGreen® Agarose LE is our ultra-pure molecular biology grade LE agarose pre-coated with GelGreen® Nucleic Acid Gel Stain. With GelGreen® Agarose, there is no need to handle concentrated fluorescent dye while preparing your gel, for greater convenience and safety.
· Non-toxic GelGreen® DNA/RNA dye is already in the agarose
· No need to handle concentrated dye solutions, for enhanced safety
· Use with TAE or TBE for 0.8% to 2% gels
· Low EEO, ultrapure molecular biology grade agarose
· Compatible with blue light illuminators
GelGreen® Agarose LE has low electroendosmosis (EEO) for high electrophoretic mobility. This agarose has excellent performance for analytical or preparative nucleic acid electrophoresis and blotting. It is suitable for preparing 0.8%-2% gels in TAE or TBE buffer. In a 1% GelGreen® Agarose gel, the final GelGreen® concentration is 1X, just like in our standard precast protocol. GelGreen® Agarose also gives excellent results at percentages between 0.8% (0.8X GelGreen®) up to 2% (2X GelGreen®).
GelGreen® is a sensitive, stable and environmentally safe green fluorescent nucleic acid dye specifically designed for gel staining. GelGreen® is far more sensitive than SYBR® Safe. Unlike SYBR® dyes, which are known to be unstable, GelGreen® is very stable, both hydrolytically and thermally. GelGreen® has UV absorption between 250 nm and 300 nm and a strong absorption peak centered around 500 nm. Thus, GelGreen® is compatible with UV transilluminators or blue light illuminators (like the Dark Reader® or Biotium’s Gel-Bright™ LED Illuminator). With blue light illuminators, researchers can avoid exposure to UV irradiation for themselves and their DNA samples, for a safer work environment and higher cloning efficiency.
Gel staining with GelGreen® is compatible with downstream applications such as sequencing and cloning. GelGreen® can be removed from DNA using a gel extraction kit, or by phenol/chloroform extraction followed by ethanol precipitation. GelGreen® can be used to stain dsDNA, ssDNA or RNA, however GelRed® is much more sensitive for staining single-stranded nucleic acids than GelGreen®. To learn more about GelGreen®, see the GelRed® & GelGreen® technology page, or FAQs (below).
GelGreen® was subjected to a series of tests at Biotium and by three independent testing services to assess the dye’s safety for routine handling and disposal. Test results confirm that the dye is impenetrable to both latex gloves and cell membranes. The dye is non-cytotoxic and non-mutagenic at concentrations well above the working concentrations used in gel staining. Using GelGreen® Agarose further minimizes risk by avoiding the need to handle concentrated dye solution. GelGreen® successfully passed environmental safety tests in compliance with CCR Title 22 Hazardous Waste Characterization, under which GelGreen® is classified as non-hazardous waste. See the GelRed® and GelGreen® Safety Report.
Dark Reader is a registered trademark of Clare Chemical. SYBR is a registered trademark of Thermo Fisher Scientific.