Mix-n-Stain small ligand labeling kits are designed for rapid labeling of low molecular weight (MW approx. 150 -5000) and relatively high affinity biological ligands (or substrates) without a purification step. The ligand to be labeled must contain an aliphatic amine group that is biologically inert. The amino group will form a covalent linkage with the provided reactive CF dyes. For example, suitable ligands or substrates include SNAP-tag, CLIP-tag and HaloTag ligand derivatives that have an aliphatic amine. For more information, please download the Mix-n-Stain small ligand labeling kit flyer and the product information sheet for this product.
This kit is for labeling the small ligand with red fluorescent CF568 for surface staining of live cells. CF568 has an excitation spectrum optimally matching the 568 nm line of the Ar-Kr mixed-gas laser. Protein conjugates of the dye are much brighter and more photostable than those of Alexa Fluor? 568.