Mix-n-Stain small ligand labeling kits are designed for rapid labeling of low molecular weight (MW approx. 150 -5000) and relatively high affinity biological ligands (or substrates) without a purification step. The ligand to be labeled must contain an aliphatic amine group that is biologically inert. The amino group will form a covalent linkage with the provided reactive CF dyes. For example, suitable ligands or substrates include SNAP-tag, CLIP-tag and HaloTag ligand derivatives that have an aliphatic amine. For more information, please download the Mix-n-Stain small ligand labeling kit flyer and the product information sheet for this product.
This kit is for labeling the small ligand with deep red fluorescent CF594 for surface staining of live cells. CF594 is spectrally similar to Alexa Fluor? 594 and Texas Red? but yields much brighter protein conjugates due to its high quantum yield and exceptional water solubility. CF594 also has excellent photostability ideal for demanding applications, such as confocal microscopy and single molecule imaging.