92360 Mix-n-Stain CF660C Small Ligand Labeling Kit
Mix-n-Stain small ligand labeling kits are designed for rapid labeling of low molecular weight (MW approx. 150 -5000) and relatively high affinity biological ligands (or substrates) without a purification step. The ligand to be labeled must contain an aliphatic amine group that is biologically inert. The amino group will form a covalent linkage with the provided reactive CF dyes. For example, suitable ligands or substrates include SNAP-tag, CLIP-tag and HaloTag ligand derivatives that have an aliphatic amine. For more information, please download the Mix-n-Stain small ligand labeling kit flyer and the product information sheet for this product.
This kit is for labeling the small ligand with far-red fluorescent CF660C for surface staining of live cells. CF660C is a cyanine-based dye engineered to be highly water-soluble without being excessively charged. As a result, CF660C-antibody conjugates have high signal-to-noise ratio in immunostaining. CF660C is several fold brighter and significantly more photostable than Alexa Fluor? 660. CF660C absorbs at 667 nm but can still be sufficiently excited by the 633 or 635 nm laser with emission at 685 nm in the borderline spectral region between far-red and near-IR fluorescence.