Mix-n-Stain small ligand labeling kits are designed for rapid labeling of low molecular weight (MW approx. 150 -5000) and relatively high affinity biological ligands (or substrates) without a purification step. The ligand to be labeled must contain an aliphatic amine group that is biologically inert. The amino group will form a covalent linkage with the provided reactive CF dyes. For example, suitable ligands or substrates include SNAP-tag, CLIP-tag and HaloTag ligand derivatives that have an aliphatic amine. For more information, please download the Mix-n-Stain small ligand labeling kit flyer and the product information sheet for this product.
This kit is for labeling the small ligand with far-red/near-infrared fluorescent CF680 for surface staining of live cells. CF680 is brighter than other spectrally similar dyes. Most importantly, antibody conjugates prepared from CF680 have by far the best signal-to-noise ratio than antibody conjugates prepared from other spectrally similar dyes. Please also see CF680R, an extremely photostable rhodamine-based far-red/near-IR dye.