This CF?568 tyramide conjugate is used for tyramide signal amplification (TSA)1,2,3, which is an enzyme-mediated detection method that uses horseradish peroxidase (HRP) to generate high-density labeling of a target protein or nucleic acid in situ. A tyramide conjugate is incubated in the presence of HRP (often bound to a secondary antibody), which catalyzes the binding of the tyramide (and associated label) to adjacent tyrosines for enhanced fluorescent signal. CF?568 has an excitation spectrum optimally matching the 568 nm line of the Ar-Kr mixed-gas laser. Conjugates of the dye are much brighter than those of Alexa Fluor? 568.
