This CF?594 tyramide conjugate is used for tyramide signal amplification (TSA)1,2,3, which is an enzyme-mediated detection method that uses horseradish peroxidase (HRP) to generate high-density labeling of a target protein or nucleic acid in situ. A tyramide conjugate is incubated in the presence of HRP (often bound to a secondary antibody), which catalyzes the binding of the tyramide (and associated label) to adjacent tyrosines for enhanced fluorescent signal. CF?594 is spectrally similar to Alexa Fluor? 594 and Texas Red? dye but yields much brighter protein conjugates due to its high quantum yield and photostability.
