H2DCFDA (2′,7′-Dichlorodihydrofluorescein diacetate) is a useful fluorogenic reagent to detect reactive oxygen intermediates in cells. On oxidation, H2DCFDA becomes the highly green fluorescent 2′,7′-dichlorofluorescein.
λEx/λ Em (pH>6) = 504/529 nm (end product)
White solid soluble in DMSO or DMF
Store desiccated at -20°C and protect from light