Ac-DEVD-AMC is a fluorogenic substrate for caspase 3 and can be used to continuously measure the activity of caspase-3 in cell extracts, using a fluorometer or fluorescence microplate reader. For real-time detection of caspase-3 activity in intact cells, see our novel NucView488 Caspase-3 Assay Kit.
λEx/λEm of substrate = 330/390 nm (weak fluorescence)
λEx/λEm of end product (AMC) = 342/441 nm (strong fluorescence)
Off-white solid soluble in DMSO