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产品信息

qPCR定量试剂盒
一抗
仪器
荧光二抗和其他偶联物

抗原标签和抗生物素标记

链霉亲和素标记

CF标记凝集素

R-PE 、 APC标记

HRP标记

CF 染料和生物素标记的二抗

其它CF产品

蛋白 抗体 小分子标记试剂盒

Mix-n-Stain 抗体标记试剂盒

Mix-n-Stain 小分子标记试剂盒

SE基团蛋白标记试剂盒

Vivobrite快速抗体标记试剂盒

酶底物

β-半乳糖苷酶底物

β-葡萄糖醛酸酶底物

萤火虫荧光素酶和海肾荧光素酶底物

蛋白酶和肽酶底物

过氧化物酶底物

磷酸酶底物

还原酶底物

其它酶底物

荧光素酶报告检测
细胞凋亡和活力检测
核酸染色和基因组产品

活性 DNA 结合染料

核酸凝胶电泳

qPCR相关产品

DNA定量试剂盒

核苷酸标记

双链DNA定量试剂盒
蛋白检测和定量
细菌革兰氏检测和活力试剂盒
活性染料和生物素衍生物

Mix-n-Stain小分子标记试剂盒

活性CF染料标记试剂盒

氨基活性基团

马来酰亚胺活性基团

MTS和TS探针

炔烃, 叠氮化物, BCN,

糖蛋白和碳水化合物标记

荧光染料标记氨基基团

光反应探针

荧光离子指示剂和相关产品

钙离子指示剂

PH指示剂

镁离子指示剂

锌离子指示剂

氯离子指示剂

膜染料和膜电位染料

膜电位染料

线粒体染料

神经末端染料

亲脂性carbocyanine染料

磷脂膜探针

其它膜染料

缓冲液和其他附属产品

联系方式

上海开放生物科技有限公司

电  话:400-630-2280

邮  编:200233

Email:info@bio-ope.com

网  址:www.bio-ope.com

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荧光素酶报告检测

30018 Apoptotic, Necrotic, and Healthy Cells Quantification Kit

30018 Apoptotic, Necrotic, and Healthy Cells Quantification Kit
  • 货号30018
  • 规格50 assays
  • 价格

详细描述

Apoptosis and necrosis are two major processes by which cells die. Apoptosis is an active, genetically regulated disassembly of the cell from within. During apoptosis, phosphatidylserine (PS) is translocated from the inner to the outer surface of the cell for phagocytic cell recognition. The human anticoagulant Annexin V is a 35 kDa, Ca2+-dependent phospholipid-binding protein with a high affinity for PS. Annexin V labeled with fluorescein (FITC) (λEx/λEm = 492/514 nm) can identify apoptotic cells in green by binding to PS exposed on the outer leaflet. Necrosis normally results from a severe cellular insult, resulting in loss of plasma membrane integrity. Ethidium homodimer III (EthD-III) is a highly positively charged nucleic acid probe, which is impermeant to live cells or apoptotic cells, but stains necrotic cells with red fluorescence (λEx/λEm (with DNA) = 528/617 nm). EthD-III is a superior alternative to propidium iodide (PI) or ethidium homodimer I because of its significantly higher affinity for DNA and higher fluorescence quantum yield. The kit also includes the membrane-permeant blue fluorescent DNA dye Hoechst 33342 (λEx/λEm (with DNA) = 350/461nm) for visualizing all cells. The kit allows simultaneous quantification of apoptotic (green), necrotic (red) and healthy cells (blue) within the same cell population by flow cytometry or fluorescence microscopy. Kit Components: FITC-Annexin V Ethidium Homodimer III Hoechst 33342 5X binding Buffer Features: Simple: Simultaneous detection of apoptotic, necrotic and healthy cells in a single assay Fast: A single 15-30 min staining step Sensitive: EthD-III is a superior alternative to both PI and EthD-I for staining necrotic cells. Also see the Apoptosis and Necrosis Quantitation Kit Plus (catalog no. 30065) and Apoptotic, Necrotic, and Healthy Cells Quantitation Kit Plus (catalog no. 30066), which feature Annexin V conjugated to Biotium’s superior green fluorescent dye CF488A, which is brighter and more photostable than FITC.
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上海开放生物科技有限公司

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上海市徐汇区虹漕路39号E座3楼

Biotium 中国区总代理-上海开放生物科技有限公司,专利产品和技术:CF染料新一代荧光标记染料,EvaGreen高分辨率溶解曲线饱和染料,NucView双功能荧光凋亡检测Caspase3/7,Cheetah热启动酶用于多重PCR技术和SNP检测、突变扫描等,AllGlo探针合成染料(替代Taqman技术),细胞凋亡、活性检测试剂盒。DNA定量试剂盒、蛋白定量试剂盒、核酸安全无毒灵敏染料GelRed、蛋白快速染色、神经、离子指示探针等等。

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