In the TUNEL assay, TdT enzyme catalyzes the addition of labeled dUTP to the 3′- ends of the internucleosomal DNA strand breaks that are a hallmark of apoptosis. The CF488 TUNEL Assay Apoptosis Detection Kit employs dUTP conjugated to the exceptionally bright and photostable CF488 green fluorescent dye, for single step fluorescent TUNEL labeling of fixed cells or tissues for analysis by fluorescence microscopy or flow cytometry.
