MitoView? 633 is a far-red fluorescent mitochondrial dye with absorbance/emission at 622/648 nm. The dye is membrane permeable and becomes brightly fluorescent upon accumulation in the mitochondrial membrane. Staining is dependent on mitochondrial membrane potential, and can be used to monitor mitochondrial membrane potential in intact cells. The dye is designed for use in live cells, and is not fixable.
Note: The optimal detection settings for MitoView? 633 are the same as for Cy?5 and other far-red dyes. However, the dye also has visible red fluorescence and can be imaged using Cy?3 settings as well. As a consequence, the dye cannot be used for two-color imaging with other red probes.
Also see MitoView? Blue, our blue fluorescent membrane potential dye, MitoView? Green, our membrane potential-independent green fluorescent mitochondrial dye, and our wide selection of other Membrane and Organelle stains.