30062 NucView 488 and MitoView 633 Apoptosis Assay Kit
This kit contains the NucView488 Caspase-3 Substrate and the MitoView?633 mitochondrial dye. The kit provides a convenient tool for profiling apoptotic cells based on caspase-3 activity and changes in the mitochondrial membrane potential using either fluorescence microscopy or flow cytometry. The bi-functional NucView488 Caspase-3 Substrate is able to detect both intracellular caspase-3 and at the same time stain the cell nucleus, which is known to undergo morphological changes during apoptosis. MitoView?633 is a new far-red fluorescent mitochondrial dye, which diffuses across the plasma membrane and accumulates in the mitochondria. The staining is dependent upon the mitochondrial membrane potential; thus, apoptotic cells exhibit a much lower MitoView?633 dye staining compared to healthy cells. Furthermore, the spectral separation of these two dyes minimizes fluorescence overlap.
Note: The optimal detection settings for MitoView? 633 are the same as for Cy?5 and other far-red dyes. However, the dye also has visible red fluorescence and can be imaged using Cy?3 settings as well. As a consequence, the dye cannot be used for two-color imaging with other red probes.
See our full selection of NucView substrates and kits. For more information about NucView, see the NucView flyer and reference list under the downloads tab, or see the NucView FAQs.
NucView enzyme substrate technology is covered by U.S. Patents.