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产品信息

qPCR定量试剂盒
一抗
仪器
荧光二抗和其他偶联物

抗原标签和抗生物素标记

链霉亲和素标记

CF标记凝集素

R-PE 、 APC标记

HRP标记

CF 染料和生物素标记的二抗

其它CF产品

蛋白 抗体 小分子标记试剂盒

Mix-n-Stain 抗体标记试剂盒

Mix-n-Stain 小分子标记试剂盒

SE基团蛋白标记试剂盒

Vivobrite快速抗体标记试剂盒

酶底物

β-半乳糖苷酶底物

β-葡萄糖醛酸酶底物

萤火虫荧光素酶和海肾荧光素酶底物

蛋白酶和肽酶底物

过氧化物酶底物

磷酸酶底物

还原酶底物

其它酶底物

荧光素酶报告检测
细胞凋亡和活力检测
核酸染色和基因组产品

活性 DNA 结合染料

核酸凝胶电泳

qPCR相关产品

DNA定量试剂盒

核苷酸标记

双链DNA定量试剂盒
蛋白检测和定量
细菌革兰氏检测和活力试剂盒
活性染料和生物素衍生物

Mix-n-Stain小分子标记试剂盒

活性CF染料标记试剂盒

氨基活性基团

马来酰亚胺活性基团

MTS和TS探针

炔烃, 叠氮化物, BCN,

糖蛋白和碳水化合物标记

荧光染料标记氨基基团

光反应探针

荧光离子指示剂和相关产品

钙离子指示剂

PH指示剂

镁离子指示剂

锌离子指示剂

氯离子指示剂

膜染料和膜电位染料

膜电位染料

线粒体染料

神经末端染料

亲脂性carbocyanine染料

磷脂膜探针

其它膜染料

缓冲液和其他附属产品

联系方式

上海开放生物科技有限公司

电  话:400-630-2280

邮  编:200233

Email:info@bio-ope.com

网  址:www.bio-ope.com

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荧光素酶报告检测

30072 NucView 488 and RedDot 2 Apoptosis and Necrosis Kit

30072 NucView 488 and RedDot 2 Apoptosis and Necrosis Kit
  • 货号30072
  • 规格100 assays
  • 价格

详细描述

NucView488 and RedDot?2 Apoptosis/Necrosis Assay Kit contains NucView488 Caspase-3 Substrate for detection of caspase-3/7 activity. The far-red dead-cell stain RedDot 2 is included for staining necrotic and late apoptotic cells that have compromised plasma membrane integrity. This kit provides a convenient tool for profiling apoptotic and necrotic cell populations by fluorescence microscopy or flow cytometry. In contrast to other fluorogenic caspase substrates or fluorescent caspase inhibitor based (FLICA) assays, NucView488 Caspase-3 Substrate can be used to detect caspase-3/7 activity within individual intact cells without inhibiting apoptosis progression. The substrate consists of a fluorogenic DNA dye coupled to the caspase-3/7 DEVD recognition sequence. The substrate, which is initially non-fluorescent, penetrates the plasma membrane and enters the cytoplasm. In apoptotic cells, caspase-3/7 cleaves the substrate, releasing the high-affinity DNA dye, which migrates to the cell nucleus and stains DNA with bright green fluorescence. Thus, NucView488 Caspase-3 Substrate allows detection caspase-3/7 activity and visualization of morphological changes in the nucleus during apoptosis. RedDot?2 is a cell membrane-impermeable, far-red dye with high selectivity for membrane compromised or dead cells. The far-red emission of RedDot?2 is well-separated from the green fluorescence of NucView488. RedDot?2 can be excited by wavelengths from 488 to 647 nm, and therefore can be used with the 488 nm flow cytometry laser line. Note: while NucView488 staining is formaldehyde-fixable and compatible with subsequent immunostaining, fixation after staining with RedDot?2 is not recommended because it can result in increased background staining of healthy cells. See our full selection of NucView substrates and kits. NucView enzyme substrate technology is covered by U.S. patents.
点击次数:  更新时间:2015-10-14 13:00:54  【打印此页】  【关闭】
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上海开放生物科技有限公司

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Biotium 中国区总代理-上海开放生物科技有限公司,专利产品和技术:CF染料新一代荧光标记染料,EvaGreen高分辨率溶解曲线饱和染料,NucView双功能荧光凋亡检测Caspase3/7,Cheetah热启动酶用于多重PCR技术和SNP检测、突变扫描等,AllGlo探针合成染料(替代Taqman技术),细胞凋亡、活性检测试剂盒。DNA定量试剂盒、蛋白定量试剂盒、核酸安全无毒灵敏染料GelRed、蛋白快速染色、神经、离子指示探针等等。

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