The AccuBlue NextGen dsDNA Quantitation Kit provides an unprecedented level of sensitivity in dsDNA detection. The AccuBlue NextGen?dsDNA Quantitation assay is the most sensitive and accurate DNA quantitation method for ultra low amounts of DNA.?With a detection limit as low as 1 pg DNA in a 96-well plate reader assay (see Table 1), AccuBlue NextGen outperforms rivals such as PicoGreen? in sensitivity and accuracy. Specially designed for scientists who need to quantify extremely dilute or precious DNA, AccuBlue NextGen is ideal for use in preparing samples for applications such as Next Generation Sequencing (NGS) and digital PCR.
The AccuBlue NextGen?dsDNA quantitation assay is designed for use with fluorescence 96-well plate readers equipped with excitation and emission filters for detecting green fluorescence. The unique spectral properties of AccuBlue NextGen Dye make it especially well-suited for use with instruments with blue LED excitation sources.?Because the AccuBlue NextGen assay is so sensitive, results will vary depending on many factors, such as microplate reader (see Table 1), assay volume, and pipet accuracy.
The AccuBlue NextGen dsDNA Quantitation Kit?contains AccuBlue NextGen Dye, Enhancer, assay buffer, and dsDNA standard, with instructions for preparing a set of standards by serial dilution.
AccuBlue NextGen Dye, 400X
AccuBlue NextGen Enhancer, 100X
AccuBlue NextGen Buffer, 1X
AccuBlue NextGen DNA Standard, 300 pg/uL
Linear range: 1-3000 pg dsDNA
Green fluorescence (Ex/Em 460/507 nm)
Green fluorescent dye is a perfect match for blue LED excitation sources
Green fluorescence detection compatible with fluorescence microplate readers, handheld fluorometers such as the AccuLite 470, and ?NanoDrop? fluorospectrophotometers.
reader assay format (assay volume) lowest DNA detected
BMG CLARIOStar 96-well (200 uL) 1 pg
BMG CLARIOStar 384-well (50 uL) 0.6 pg
Biotek Synergy H1m 96-well (200 uL) 5 pg
SpectraMax Gemini XS 96-well (200 uL) 5 pg
AccuLite 470 PCR tube (200 uL) 5 pg
Please also see related products for information on our other dsDNA Quantitation Kits such as AccuClear Ultra High Sensitivity dsDNA Quantitation Kit (.03-250 ng), AccuBlue High Sensitivity dsDNA Quantitation Kit?or Solution (0.2-100 ng) and AccuBlue Broad Range dsDNA Quantiation Kit or Solution (2-2000 ng).
AccuBlue NextGen technology is covered by U.S. patents.