31027-T AccuClear Ultra High Sensitivity dsDNA Quantitation Solution
AccuClear Ultra High Sensitivity dsDNA Quantitation Solution utilizes a novel, next-generation DNA binding dye for highly sensitive and accurate quantitation of purified dsDNA samples across a broad range of DNA concentrations. The assay is linear between 0.03 ng and 250 ng of dsDNA per assay in microplate format. Unlike absorbance-based measurements, AccuClear dye is highly selective for double-stranded DNA over single stranded DNA or RNA.
The AccuClear dsDNA quantitation assay is designed for fluorescence 96-well plate readers equipped with excitation and emission filters for detecting green fluorescence. The unique spectral properties of AccuClear dye make it especially well-suited for use with instruments with blue LED excitation sources. Biotium’s AccuLite? 470 Mini Fluorometer (cat. no. E90001) includes a program for use with the AccuClear assay. AccuClear also is compatible with handheld fluorometers such as Invitrogen’s Qubit? and Promega’s QuantiFluor?-P, however the standard curve calibration programs for these instruments may not cover the full dynamic range of the AccuClear kit standard curve.
The AccuClear Ultra High Sensitivity dsDNA Quantitation Solution includes AccuClear dye and assay buffer. Biotium also offers the AccuClear Ultra High Sensitivity dsDNA Quantitation Kit (catalog no. 31028), which includes dye, buffer, and a set of pre-diluted calf thymus dsDNA standards. For large numbers of samples, Biotium offers the AccuClear Ultra High Sensitivity dsDNA Quantitation Kit with 1 DNA Standard (2000 assays, cat. no. 31029) which includes dye, 20X assay buffer, and a 25 ng/uL DNA standard stock solution that can be used to prepare a range of standards.
Linear range: 0.03-250 ng dsDNA
Green fluorescence (Ex/Em 460/507 nm)
Unrivaled sensitivity and dynamic range
Novel green fluorescent dye is a perfect match for blue LED excitation sources
Green fluorescence detection compatible with fluorescence microplate reader, handheld fluorometer, or NanoDrop? fluorospectrophotometer.
Please also see related products for information on the AccuBlue High Sensitivity dsDNA Quantitation Solution and Kit (0.2-100 ng) and AccuBlue Broad Range dsDNA Quantiation Solution and Kit (2-2000 ng), In addition to different linear ranges, these assays have different tolerances to various contaminants compared to AccuClear (see product protocols for details).
AccuClear kits are intended for quantitation of purified dsDNA samples. For cell quantitation assays, please see our cell viability and cytotoxicity assays.
AccuClear technology is covered by pending U.S. and international patents.