Lumitein is a luminescent dye designed for detecting proteins in SDS polyacrylamide (SDS-PAGE) gels, but that can also be used to detect proteins in native PAGE gels after an additional SDS incubation step. The stain combines excellent sensitivity, exceptional user-friendliness and compatibility with common instruments and downstream analysis. Lumitein protein gel stain is supplied as a highly concentrated 100X solution, which provides convenience for shipping, handling and storage. More importantly, the concentrated solution is economical because of reduced manufacturing and shipping costs. Lumitein is also available as a convenient ready-to-use 1X staining solution (catalog no. 21001). FeaturesHighly Sensitive: At least as sensitive as silver stain by detecting < 1 ng protein. Simple & Fast: Fixation and staining in a single 30-90 minute step, followed by a simple rinse in water. Very Low Background Instrument Compatibility: Image with 300 nm UV gel box (EtBr filter), Dark Reader, or a laser scanner. Wide Linear Detection Range: At least three orders of magnitude. Compatible with Downstream Analysis: Compatible with MS and sequencing. Highly Stable: Both 100X and 1X solutions are stable at room temperature. Economical: 100X concentrate with reduced manufacturing and shipping costs
Figure 1. Two-fold serial dilutions of protein marker were separated via SDS-PAGE and then stained with Lumitein. Imaged with a GE Typhoon Trio using 532 nm excitation and 610BP30 emission filter.Figure 2. 2-D gel of human liver protein lysate stained with Lumitein. The three circled spots were picked for MS analysis by Applied Biomics, Inc. (Hayward, CA), confirming that Lumitein staining is fully compatible with MS analysis.
Lumitein vs. Coomassie
Figure 3. PAGE gels containing electrophoretically separated protein marker (loaded in two-fold serial dilution from left to right) were stained with Lumitein total protein gel stain (left) and Coomassie Blue (right), respectively. Lumitein-stained gel was imaged using a UV box equipped with EtBr filter (UVP) while the Coomassie-stained gel was imaged using a white light converter (UVP).