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产品信息

qPCR定量试剂盒
一抗
仪器
荧光二抗和其他偶联物

抗原标签和抗生物素标记

链霉亲和素标记

CF标记凝集素

R-PE 、 APC标记

HRP标记

CF 染料和生物素标记的二抗

其它CF产品

蛋白 抗体 小分子标记试剂盒

Mix-n-Stain 抗体标记试剂盒

Mix-n-Stain 小分子标记试剂盒

SE基团蛋白标记试剂盒

Vivobrite快速抗体标记试剂盒

酶底物

β-半乳糖苷酶底物

β-葡萄糖醛酸酶底物

萤火虫荧光素酶和海肾荧光素酶底物

蛋白酶和肽酶底物

过氧化物酶底物

磷酸酶底物

还原酶底物

其它酶底物

荧光素酶报告检测
细胞凋亡和活力检测
核酸染色和基因组产品

活性 DNA 结合染料

核酸凝胶电泳

qPCR相关产品

DNA定量试剂盒

核苷酸标记

双链DNA定量试剂盒
蛋白检测和定量
细菌革兰氏检测和活力试剂盒
活性染料和生物素衍生物

Mix-n-Stain小分子标记试剂盒

活性CF染料标记试剂盒

氨基活性基团

马来酰亚胺活性基团

MTS和TS探针

炔烃, 叠氮化物, BCN,

糖蛋白和碳水化合物标记

荧光染料标记氨基基团

光反应探针

荧光离子指示剂和相关产品

钙离子指示剂

PH指示剂

镁离子指示剂

锌离子指示剂

氯离子指示剂

膜染料和膜电位染料

膜电位染料

线粒体染料

神经末端染料

亲脂性carbocyanine染料

磷脂膜探针

其它膜染料

缓冲液和其他附属产品

联系方式

上海开放生物科技有限公司

电  话:400-630-2280

邮  编:200233

Email:info@bio-ope.com

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细菌革兰氏检测和活力试剂盒

40013 PMA dye

40013 PMA dye
  • 货号40013
  • 规格1 mg
  • 价格

详细描述

下载中心:


Flyer (PDF): FL-PMA-and-PMAxx-2015

MSDS (PDF): MSDS 40013_030712

Protocol (PDF): PI-40013, 40019

Reference List (PDF): PMA-References

Product Description

PMA™ (propidium monoazide) is a high affinity photoreactive DNA binding dye invented by scientists at Biotium. The dye is weakly fluorescent by itself but becomes more fluorescent after binding to nucleic acids. It preferentially binds to dsDNA with high affinity. Upon photolysis, the photoreactive azido group on the dye is converted to a highly reactive nitrene radical, which readily reacts with any hydrocarbon moiety at the binding site to form a stable covalent nitrogen-carbon bond, thus resulting in permanent DNA modification. The dye is nearly completely cell membrane-impermeable, and thus can be selectively used to modify only exposed DNA from dead cells while leaving DNA from viable cells intact. This feature makes the dye highly useful in the selective detection of viable pathogenic cells by quantitative real-time PCR in the presence dead cells whose DNA has been PMA-modified and thus can not be amplified. Since Biotium first developed PMA dye, there have been numerous publications on the use of the dye in pathogenic bacterial detection related to food and water safety, medical diagnosis and biodefense; download the PMA Reference List. PMA dye is also available in H2O (40019), a convenient ready-to-use format.

Note: Scientists at Biotium have recently invented a new and improved dye for viability PCR, called PMAxx (40069). PMAxx functions in a manner very similar to PMA, but has much greater activity and ability to distinguish between live and dead bacteria. PMAxx can be used as a replacement for PMA in any bacterial viability-PCR assay. We recommend that customers interested in using PMA try PMAxx instead, for improved detection of viable bacteria.

For viability PCR of gram-negative bacteria we highly recommend using our new PMA Enhancer (31038) in conjunction with PMA. PMA Enhancer for Gram Negative Bacteria was designed to improve PMA-mediated discrimination between live and dead gram-negative bacteria. When a sequence from a gram-negative bacteria is amplified by PCR, samples pre-treated with Enhancer show a decrease in the signal from dead cells, with no change in the signal from live cells.

Biotium also provides strain-specific PMA Real-Time PCR Bacterial Viability kits with validated primers for 7 selected pathogens: M. tuberculosis, S. aureus, MRSA, Salmonella, E. coli, E. coli O157:H7 and Listeria. These kits provide everything that you need for the selective detection of your favorite species of live bacteria by real-time PCR. Don’t see your favorite strain? Let us know attechsupport@biotium.com.


Real-Time PCR Bacterial Viability Kits

For photoactivation of PMA or EMA dye, we recommend the use of Biotium’s PMA-Lite LED Photolysis Device (E90002), which is designed to conduct photolysis under controlled conditions.

PMA diagram

Principal of selective detection of live cells using PMA dye.

  • Molecular weight: 511

  • Abs = 464 nm (before photolysis)

  • Abs/Em = 510/610 nm (following photolysis and covalent attachment to DNA/RNA)

  • Dark red solid soluble in DMSO or DMF

  • Store at 4°C and protect from light at all times


点击次数:  更新时间:2015-11-03 15:44:41  【打印此页】  【关闭】
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Biotium 中国区总代理-上海开放生物科技有限公司,专利产品和技术:CF染料新一代荧光标记染料,EvaGreen高分辨率溶解曲线饱和染料,NucView双功能荧光凋亡检测Caspase3/7,Cheetah热启动酶用于多重PCR技术和SNP检测、突变扫描等,AllGlo探针合成染料(替代Taqman技术),细胞凋亡、活性检测试剂盒。DNA定量试剂盒、蛋白定量试剂盒、核酸安全无毒灵敏染料GelRed、蛋白快速染色、神经、离子指示探针等等。

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