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qPCR定量试剂盒
一抗
仪器
荧光二抗和其他偶联物

抗原标签和抗生物素标记

链霉亲和素标记

CF标记凝集素

R-PE 、 APC标记

HRP标记

CF 染料和生物素标记的二抗

其它CF产品

蛋白 抗体 小分子标记试剂盒

Mix-n-Stain 抗体标记试剂盒

Mix-n-Stain 小分子标记试剂盒

SE基团蛋白标记试剂盒

Vivobrite快速抗体标记试剂盒

酶底物

β-半乳糖苷酶底物

β-葡萄糖醛酸酶底物

萤火虫荧光素酶和海肾荧光素酶底物

蛋白酶和肽酶底物

过氧化物酶底物

磷酸酶底物

还原酶底物

其它酶底物

荧光素酶报告检测
细胞凋亡和活力检测
核酸染色和基因组产品

活性 DNA 结合染料

核酸凝胶电泳

qPCR相关产品

DNA定量试剂盒

核苷酸标记

双链DNA定量试剂盒
蛋白检测和定量
细菌革兰氏检测和活力试剂盒
活性染料和生物素衍生物

Mix-n-Stain小分子标记试剂盒

活性CF染料标记试剂盒

氨基活性基团

马来酰亚胺活性基团

MTS和TS探针

炔烃, 叠氮化物, BCN,

糖蛋白和碳水化合物标记

荧光染料标记氨基基团

光反应探针

荧光离子指示剂和相关产品

钙离子指示剂

PH指示剂

镁离子指示剂

锌离子指示剂

氯离子指示剂

膜染料和膜电位染料

膜电位染料

线粒体染料

神经末端染料

亲脂性carbocyanine染料

磷脂膜探针

其它膜染料

缓冲液和其他附属产品

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细菌革兰氏检测和活力试剂盒

31051 PMA Real-Time PCR Bacterial Viability Kit – Listeria monocytogenes (hly)

31051 PMA Real-Time PCR Bacterial Viability Kit – Listeria monocytogenes (hly)
  • 货号31051
  • 规格200 assays
  • 价格

详细描述

PMA-PCR kits are designed for selective detection of viable bacteria from a specific strain using PMA dye and real-time PCR. The kits contain PMA dye, Fast EvaGreen qPCR Master Mix, and PCR primers for detection of selected strains of bacteria that are of widespread interest to food safety, public health, and/or antibacterial research.This kit contains primers for amplification of Listeria monocytogenes hly gene, with reagents sufficient to treat 80 bacterial cultures with PMA and perform 200 PCR reactions. The number of samples that can be treated with PMA using the kit may vary depending on sample type. See the product protocol under the downloads tab and references for more information.Kit contents:PMA dye, 20 mM in water, 100 uL 5X PMA Enhancer for Gram Negative Bacteria, 16 mL 2X Fast EvaGreen qPCR Master Mix, 2 x 1 mL (200 reactions) 10X ROX reference dye, 1 mL hly primer mix, 5 uM, 400 uL PMA? is a high affinity photoreactive DNA binding dye developed by Biotium. The dye is weakly fluorescent by itself but becomes highly fluorescent upon binding to nucleic acids. It preferentially binds to dsDNA with high affinity. Upon photolysis, the photoreactive azido group on the dye is converted to a highly reactive nitrene radical, which readily reacts with any hydrocarbon moiety at the binding site to form a stable covalent nitrogen-carbon bond, thus resulting in permanent DNA modification. The dye is cell membrane-impermeable and thus can be used to selectively modify DNA from dead cells with compromised membrane integrity, while leaving DNA from viable cells intact. PMA inhibits PCR amplification of modified DNA templates by a combination of removal of modified DNA during purification and inhibition of template amplification by DNA polymerases (see Reference 1 under the references tab). Consequently the dye is useful in the selective detection of viable pathogenic cells by quantitative real-time PCR.Fast Evagreen Master Mix is a ready-to-use hot-start mix for qPCR and DNA melt curve analysis of PCR amplicons. It is formulated for qPCR using a fast cycling protocol, but also can be used for qPCR using regular cycling protocols. EvaGreen dye is a unique DNA-binding dye with features ideal for both qPCR and melt curve analysis. EvaGreen dye binds to dsDNA via a novel “release-on-demand” mechanism, which permits the use of a relatively high dye concentration in qPCR without PCR inhibition. Fast EvaGreen Master Mix contains Cheetah? Taq, Biotium’s fast-activating chemically-modified hot-start Taq polymerase, which is particularly suitable for fast PCR cycling protocols.Listeria monocytogenes is a food-borne pathogen that can cause digestive illness and fetal harm. The gene hly encodes the Listeria Lysin O (LLO) protein that is specific for Listeria. PMA-PCR using these primers for viability PCR in Listeria has been published (see Reference 2 under the references tab). In addition, these primers have been validated at Biotium for real-time qPCR using EvaGreen Master Mix (see product protocol under downloads for details).Also see our other PMA-PCR kits for detection of Staphylococcus aureus, methicillin-resistant Staphylococcus aureus (MRSA), E. coli, E. coli O157:H7, Mycobacterium tuberculosis, and Salmonella enterica. Don’t see your favorite strain? Let us know at techsupport@biotium.com.Materials from Biotium are sold for research use only.
点击次数:  更新时间:2015-10-14 13:39:18  【打印此页】  【关闭】
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Biotium 中国区总代理-上海开放生物科技有限公司,专利产品和技术:CF染料新一代荧光标记染料,EvaGreen高分辨率溶解曲线饱和染料,NucView双功能荧光凋亡检测Caspase3/7,Cheetah热启动酶用于多重PCR技术和SNP检测、突变扫描等,AllGlo探针合成染料(替代Taqman技术),细胞凋亡、活性检测试剂盒。DNA定量试剂盒、蛋白定量试剂盒、核酸安全无毒灵敏染料GelRed、蛋白快速染色、神经、离子指示探针等等。

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